Storage of biological tissues such as blood, organs, semen reires low temperature which is achieved upon the usage of

The storage of biological tissues such as blood, organs, and semen at extremely low temperatures is known as cryopreservation. This process aims to bring biological materials to a state of 'absolute quiescence' where metabolic and chemical reactions are halted. While solid carbon dioxide (dry ice) provides temperatures around -78°C to -80°C and is often used for the transport of organs and samples [1], liquid nitrogen is the most commonly used medium for long-term storage [1]. Liquid nitrogen achieves a much lower temperature of -196°C, ensuring that cells remain in a completely inactive state for perpetual conservation [1]. This technique is essential for germplasm storage, including the freezing of sperm and eggs in zoological freezers or biobanks [1]. Other cryogens like liquid helium exist but are typically used for applications requiring temperatures closer to absolute zero rather than standard biological tissue preservation.

Sources

1. [1] https://cmpcollege.ac.in/wp-content/uploads/2020/05/e-study-Cryopreservation-and-germplasm-storage-converted.pdf