Consider the following stages of insulin production procedure through gene technology :
1. Isolation of insulin coding gene from humans
2. Inserting the insulin coding gene into a plasmid vector
3. Inserting the recombinant plasmid vector into the bacterium (E. coli )
Which of the following is a correct sequence of the above?

The production of human insulin (Humulin) using recombinant DNA technology follows a specific logical sequence in biotechnology. The process begins with the isolation of the specific gene sequence that codes for human insulin (Step 1). Once isolated, this gene is inserted into a plasmid vector (a small, circular DNA molecule) using restriction enzymes and DNA ligase to create a recombinant DNA molecule (Step 2). Finally, this recombinant plasmid is transformed (inserted) into a host bacterium, typically Escherichia coli (Step 3), which then replicates and expresses the gene to produce the insulin protein. Therefore, the correct chronological sequence is 1-2-3.