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Q117 (CDS-II/2026) Science & Technology › Biotechnology & Health

Which of the following statements is/are associated with role of enzymes in genetic engineering ?

1. Exonucleases remove nucleotides from the ends of DNA

2. Restriction endonucleases are used to form recombinant molecule of DNA

Select the answer using the code given below :

Result
Your answer: —  ·  Correct: C

Explanation

Both statements are correct regarding the role of enzymes in genetic engineering:

  • Statement 1 is correct: Nucleases are enzymes that degrade nucleic acids. Exonucleases specifically remove nucleotides one at a time from the terminal ends (either 5' or 3') of a DNA molecule.
  • Statement 2 is correct: Restriction endonucleases (often called 'molecular scissors') recognize specific nucleotide sequences and cut DNA at internal positions. By cutting DNA from different sources with the same restriction enzyme, complementary 'sticky ends' are produced. These fragments can then be joined together using DNA ligase to form a recombinant DNA molecule.

These enzymes are fundamental tools in biotechnology for gene cloning and DNA manipulation.

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