To be recognized as a `National Party` by the Election Commission of India, a political party must be treated as a recognized political party in at least three States. This means that the party should have enough presence and support in at least three states to be considered for national recognition.

Let`s dissect the other options for better understanding:

Option 1: At least two States - This is incorrect as the requirement for national party recognition is a minimum of three states.

Option 3: At least four States - This is not the correct answer as well. The requirement is three states, not four.

Option 4: At least five States - This is also not the correct answer. The requirement is three states, not five.

Therefore, the correct answer is option 3: At least three States. It is important for a political party to have a significant presence in multiple states to be recognized as a national party by the Election Commission of India.