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Q1 (CDS-II/2008) Science & Technology › Biotechnology & Health › Genomics and DNA technologies Answer Verified

By using which one of the following techniques, is DNA fingerprint done ?

Result
Your answer: —  ·  Correct: D
Explanation

DNA fingerprinting, also known as DNA profiling, primarily utilizes the Southern Blotting technique to identify unique genetic patterns. This method involves digesting DNA with restriction enzymes, separating the resulting fragments via gel electrophoresis, and transferring them to a membrane where labeled probes hybridize with specific repetitive sequences [t3, t6]. These probes target Variable Number Tandem Repeats (VNTRs) or Restriction Fragment Length Polymorphisms (RFLPs) to create a unique "fingerprint" used in criminal investigations and paternity tests [t6, t9]. In contrast, Northern Blotting is used for RNA detection [t1, t4], while ELISA (Enzyme-Linked Immunosorbent Assay) and RIA (Radioimmunoassay) are immunoassays used to detect and quantify proteins, antigens, or antibodies rather than genomic profiling [t2, t7, t8]. Southern Blotting remains a foundational molecular biology technique for DNA detection and analysis [t1, t5].

Sources

  1. [1] https://www.sciencedirect.com/science/article/abs/pii/S0022175999001702
  2. [2] https://pmc.ncbi.nlm.nih.gov/articles/PMC12133488/
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SIMILAR QUESTIONS

CAPF · 2011 · Q3 Relevance score: 3.33

Statement I : In forensic investigations, DNA fingerprinting tests are carried out. Statement I : In DNA fingerprinting technique, DNA is isolated and subjected to Northern Blotting.

IAS · 1997 · Q63 Relevance score: -1.05

Which one of the following techniques can be used to establish the paternity of a child ?

IAS · 2014 · Q66 Relevance score: -4.16

In addition to fingerprint scanning, which of the following can be used in the biometric identification of a person? 1. Iris scanning 2. Retinal scanning 3. Voice recognition Select the correct answer using the code given below.

IAS · 2000 · Q128 Relevance score: -4.47

Assertion (A) : “DNA Finger- printing” has become a powerful tool to establish paternity and identity of criminals in rape and assault cases. Reason (R) : Trace evidences such as hairs, saliva and dried semen are adequate for DNA analysis.