The correct answer is option 4: Vacuoles are absent in animal cells.

Option 1 states that in plants, there is a large central vacuole that may occupy 90% of the total cell volume. This statement is correct. Plant cells have a large central vacuole that helps maintain the cell`s shape and stores water, nutrients, and waste products.

Option 2 states that in plant cells, vacuoles provide turgidity and rigidity. This statement is correct. Vacuoles in plant cells help maintain turgidity, which is the stiffness of the cell that gives plants their rigidity. The water stored in the vacuoles creates hydrostatic pressure, keeping the cell rigid.

Option 3 states that in Amoeba, vacuoles have a role in nutrition. This statement is correct. In Amoeba, vacuoles are involved in the process of phagocytosis, where food particles are engulfed and digested. Vacuoles help in storing and breaking down food particles for energy production.

Therefore, option 4 is incorrect because vacuoles are not absent in animal cells. Animal cells also have vacuoles, although they are smaller and less prominent compared to the large central