Question map
With reference to the recent developments in science, which one of the following statements is not correct?
Explanation
The correct answer is option A. While artificial gene synthesis processes can be scaled up to create functional genes, multi-gene circuits[1] or even entire synthetic chromosomes or genomes[1], and methods exist for cloning segments of natural chromosomes and programmably assembling them into synthetic chromosomes[2], these processes work with DNA from the same or compatible species. Creating functional chromosomes by joining segments from cells of **different species** is not a standard achievable practice, as cross-species chromosomal assembly faces significant incompatibility issues.
The other options are correct: Functional genes and synthetic chromosomes can be created in laboratories[1] (Option B). DNA amplification allows multiplication of DNA pieces in test tubes into millions of copies, most commonly using the polymerase chain reaction (PCR) system[3] (Option C). In tissue culture, cells can be cultured in vitro (in glass vessels) where they undergo division and development[5] (Option D).
Sources- [1] https://en.wikipedia.org/wiki/Artificial_gene_synthesis
- [2] https://pmc.ncbi.nlm.nih.gov/articles/PMC10733283/
- [3] https://openknowledge.fao.org/server/api/core/bitstreams/44b59eaf-ec5d-4773-98ee-a20d02b4b604/content
- [4] https://openknowledge.fao.org/server/api/core/bitstreams/44b59eaf-ec5d-4773-98ee-a20d02b4b604/content
- [5] https://openknowledge.fao.org/server/api/core/bitstreams/44b59eaf-ec5d-4773-98ee-a20d02b4b604/content
PROVENANCE & STUDY PATTERN
Full viewThis is a 'Wolf in Sheep's Clothing'. It masquerades as a high-tech Current Affairs question, but Options B, C, and D are actually standard static concepts found in NCERT Class 12 Biology (Biotechnology unit). You solve this by validating the basics you know (PCR, Tissue Culture), not by researching obscure synthetic biology papers.
This question can be broken into the following sub-statements. Tap a statement sentence to jump into its detailed analysis.
- Statement 1: In recent developments in science, have researchers been able to create functional chromosomes by joining segments of DNA taken from cells of different species?
- Statement 2: In recent developments in science, can pieces of artificial functional DNA be synthesized or created in laboratories?
- Statement 3: In recent developments in science, can a piece of DNA extracted from an animal cell be made to replicate outside a living cell under laboratory conditions?
- Statement 4: In recent developments in science, can cells taken from plants and animals be induced to undergo cell division in laboratory petri dishes (in vitro)?
Defines chromosomes as separate independent pieces of DNA and notes cells carry two copies (one from each parent), highlighting DNA is organised in modular units.
A student could infer that because chromosomes are discrete units, one might test whether joining segments from different sources could form a complete, functioning chromosome and then check compatibility across species.
Explains that reproduction involves combining DNA from two individuals and that maintaining correct DNA amount and control is critical.
Use the rule that genome dosage and control matters to assess whether chimeric chromosomes from different species would function without disrupting cellular control mechanisms.
Describes a gene as a DNA section encoding a protein, emphasising functional modularity at the gene level.
From gene modularity, a student could reason that assembling segments that contain intact genes might produce specific protein functions, but must consider regulatory context and species-specific expression.
- Explicitly states short DNA oligonucleotides (primers) are synthesized in the laboratory.
- Shows that synthetic DNA pieces (β20 nucleotides) are produced for sequencing and experimental use, supporting that artificial DNA can be made.
- Describes laboratory methods that use DNA introduced into cells (DNA-coated particles), indicating practical handling and use of exogenous DNA in lab settings.
- Implies that DNA material is prepared and applied experimentally to create genetic modifications in cells.
- Refers to genetic manipulation of organisms carried out in laboratories and greenhouses, placing DNA modification/engineering squarely in lab research.
- Connects laboratory containment and research practices with techniques that alter organism genomes, implying creation or use of artificial genetic material.
Defines genetically modified organisms as ones whose hereditary material (DNA) is altered in a manner that does not occur by normal mating, and says foreign genes are artificially inserted into a plant's genes.
A student could combine this with basic knowledge that inserting genes requires creating or handling DNA sequences in the lab, suggesting laboratory creation/manipulation of functional DNA segments.
States that cells use chemical reactions to build copies of their DNA, framing DNA as a molecule assembled by chemistry.
Knowing DNA is a chemical polymer, a student could infer that chemical or biochemical synthesis methods might be used in labs to assemble DNA pieces.
Notes that DNA copying is a biochemical process prone to small inaccuracies that create variations.
A student might extend this to the idea that deliberate changes (rather than accidental ones) can be introduced using laboratory techniques to create variant DNA sequences with functional effects.
Describes efforts to build a DNA barcode reference library and facilities for sequencing and preserving DNA extracts.
Given widespread sequencing and preservation infrastructure, a student could reason labs have the data and tools needed to design and then synthesize tested DNA sequences.
- Defines DNA amplification as multiplication of a piece of DNA in a test-tube, directly stating in vitro replication is possible.
- Names PCR and other cell-free systems as methods to make many millions of copies outside living cells.
- Describes site-directed mutagenesis as changes introduced into a piece of DNA using recombinant DNA methods, indicating manipulation of isolated DNA outside cells.
- Shows molecular techniques exist to alter and work with DNA fragments in laboratory (supporting feasibility of ex vivo handling and replication workflows).
States that DNA copying is a basic event in reproduction and that cells use chemical reactions to build copies of their DNA.
A student can take the rule that DNA replication depends on chemical reactions and ask whether those reactions (enzymes, substrates, conditions) can be reproduced in a lab environment outside a whole living cell.
Explicitly notes that DNA copying is accompanied by creation/maintenance of an organised cellular apparatus and that simply having a DNA copy outside would lack that apparatus.
One could use this to reason that replication outside cells would require substituting or reconstructing the necessary cellular apparatus or its functions in vitro to enable copying.
Emphasises that DNA copying mechanisms operate inside cells and that copying is not perfectly accurate (errors arise from biochemical reactions).
A student could use this to consider whether isolated DNA in lab conditions would need the same biochemical machinery and whether fidelity of copying might differ outside cellular contexts.
Summarises that reproduction involves creation of a DNA copy and additional cellular apparatus by the cell involved in the process.
This summary supports asking if laboratory methods must provide both the copying chemistry and some form of supporting apparatus (enzymatic systems, controlled environment) to replicate DNA outside cells.
Describes regeneration where specialised cells proliferate and generate many cellsβshowing that DNA copying is tied to cellular proliferation performed by specialised cell machinery.
A student could infer that DNA replication in vitro would need to mimic specialised cellular proliferation machinery or provide the key components those cells use.
- Explicitly states plant growth regulators (cytokinins) are substances that induce cell division and differentiation.
- Links those regulators to tissue culture outcomes (callus and shoot development), showing induction of division in vitro.
- Defines 'in vitro' as living in test tubes or artificial vessels where cultured cells, tissues or whole plants reside.
- Establishes the laboratory context (glass vessels/petri-dish-like environments) for inducing cell behavior outside the organism.
- Defines totipotency as the property of somatic cells to be induced to undergo regeneration.
- Implies that cells taken from organisms can be stimulated (in experimental settings) to divide and regenerate different cell types.
Describes tissue culture where cells removed from a plant are placed in an artificial medium and 'divide rapidly to form a small group of cells or callus.'
A student could infer that providing artificial medium and hormones can induce plant cells removed from a plant to divide in laboratory conditions (in vitro) and test this by attempting plant tissue culture in a sterile medium with growth regulators.
Explains regeneration: specialised cells proliferate and make large numbers of cells, which then differentiate into tissues.
One could extend this pattern to hypothesise that certain differentiated animal cells (or specialised regenerative cells) can be stimulated to proliferate outside the organism if given appropriate signals or environment and then design cell culture experiments to test proliferation.
Notes that unicellular organisms undergo cell division (fission) naturally, showing that cells inherently possess division mechanisms.
Combine this basic rule with the idea that if single cells divide naturally, providing suitable lab conditions (nutrients, sterile environment) might allow isolated cells to divide in vitro, so one could attempt to culture isolated single cells to observe division.
Describes cell division processes (meiosis) in germ cells as a defined cellular mechanism for producing new cells.
A student can infer that since cell division is a definable cellular program (mitosis/meiosis), it may be triggered or supported in isolated cells under controlled lab conditions (e.g., by supplying energy/nutrients or appropriate cues) and plan experiments to culture germ or somatic cells to observe division.
Shows that plant cells (onion peel) are readily observable under microscope and exist as intact units, implying they can be isolated and studied.
A learner might extend this by isolating visible plant cells and attempting to culture them on media to see whether they undergo division in vitro.
- [THE VERDICT]: Sitter disguised as a Bouncer. Solvable purely via NCERT Class 12 Biology (Biotechnology Principles).
- [THE CONCEPTUAL TRIGGER]: Biotechnology & Applications. Specifically: PCR (Polymerase Chain Reaction) and Tissue Culture.
- [THE HORIZONTAL EXPANSION]: 1. PCR steps (Denaturation, Annealing, Extension). 2. Totipotency (Plant cells) vs Pluripotency (Stem cells). 3. Somatic Hybridization (Protoplast fusion, e.g., Pomato). 4. Three-parent baby (Mitochondrial replacement). 5. CAR-T Cell Therapy (modifying T-cells).
- [THE STRATEGIC METACOGNITION]: When a question asks 'Which is NOT correct' about 'Recent Developments', look for the options that are definitely TRUE based on static textbooks. Option C is PCR. Option D is Tissue Culture. Option B is basic Primer Synthesis. By elimination, A is the outlier.
Chromosomes are separate, independent pieces of DNA that carry genes and are inherited as units.
High-yield for questions on inheritance and Mendelian genetics; connects concepts of genes, karyotypes, and how traits are transmitted across generations. Mastery helps answer questions about chromosomal basis of traits and chromosomal abnormalities.
- Science , class X (NCERT 2025 ed.) > Chapter 8: Heredity > separate traits, shape and colour of seeds Figure 8.5 > p. 132
Sexual reproduction uses specialised germ cells with half the number of chromosomes so that fusion of two gametes restores the species' normal chromosome count.
Critical for understanding meiosis, fertilisation, ploidy, and why sexual reproduction prevents genome doubling each generation. Useful for questions on reproductive biology, life cycles, and genetic stability.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.3.1 Why the Sexual Mode of Reproduction? > p. 120
- Science , class X (NCERT 2025 ed.) > Chapter 8: Heredity > separate traits, shape and colour of seeds Figure 8.5 > p. 132
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.3.3 Reproduction in Human Beings > p. 122
DNA copying is not perfectly accurate; occasional errors during replication generate variations within populations.
Central to topics on mutation, evolution, and heredity; explains origin of variation that natural selection acts on and underpins questions on evolutionary mechanisms and genetic disorders.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.3.1 Why the Sexual Mode of Reproduction? > p. 119
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.1 DO ORGANISMS CREATE EXACT COPIES OF THEMSEL THEMSELVES? > p. 113
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.1 DO ORGANISMS CREATE EXACT COPIES OF THEMSEL THEMSELVES? > p. 114
DNA copying is a chemical process that produces near-identical copies but introduces small variations that affect heredity and diversity.
High-yield: explains mutation, inheritance patterns and the origin of biological variation; links to evolution, breeding and genetic disorders. Mastering this helps answer questions on causes of variation, fidelity of replication and implications for population genetics.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.1 DO ORGANISMS CREATE EXACT COPIES OF THEMSEL THEMSELVES? > p. 113
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.1 DO ORGANISMS CREATE EXACT COPIES OF THEMSEL THEMSELVES? > p. 114
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > What you have learnt > p. 126
Hereditary material can be altered by artificially inserting a foreign gene (transgene) into an organism's genome.
High-yield: central to biotechnology and GM crop policy debates; connects to agriculture, biosafety, regulation and socio-economic impacts. Useful for questions on methods, benefits, risks and governance of gene technology.
- Indian Economy, Nitin Singhania .(ed 2nd 2021-22) > Chapter 9: Agriculture > GENETICALLY MODIFIED (GM) CROPS > p. 301
Techniques exist to sequence, catalogue and preserve DNA extracts for species identification and biosurveillance.
High-yield: relevant to biodiversity assessment, conservation strategy and forensic identification; connects to large-scale databases, biosurveillance infrastructure and national biodiversity missions. Enables questions on technological capability and conservation policy.
- Environment, Shankar IAS Acedemy .(ed 10th) > Chapter 16: Conservation Efforts > Goals: > p. 249
DNA copying is carried out by biochemical reactions that operate within the cell.
High-yield for questions on cell biology and biotechnology basics: distinguishes in vivo cellular replication from laboratory techniques and frames limits of simple extrapolation. Connects to topics on molecular biology methods, ethical debates, and biosciences policy in GS papers.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.1 DO ORGANISMS CREATE EXACT COPIES OF THEMSEL THEMSELVES? > p. 113
- Science ,Class VIII . NCERT(Revised ed 2025) > Chapter 2: The Invisible Living World: Beyond Our Naked Eye > A step further > p. 13
Somatic Hybridization (Protoplast Fusion). While creating a 'functional chromosome' from different species is false (Option A), fusing entire cells from different species (e.g., Potato + Tomato) is possible but often results in sterile or unstable hybrids. UPSC loves testing the limits of 'mixing' species.
The 'Complexity vs. Compatibility' Heuristic. Options B, C, and D describe manipulating *components* (DNA pieces, single cells). Option A describes creating a highly complex *structure* (Chromosome) from *incompatible sources* (different species). In biology, 'different species' usually implies a barrier. Nature hates chimeras at the chromosomal level. Bet against the option that claims to easily overcome species barriers.
GS-3 Internal Security & Disaster Management: Link Option B (Artificial DNA) to 'Biosecurity' and 'Dual-use Research'. If artificial DNA can be created in labs, it raises the threat of engineered pathogens (Bioterrorism) and the need for the Biological Weapons Convention (BWC).