Question map
'Microsatellite DNA' is used in the case of which one of the following?
Explanation
The correct answer is Option 1: Studying the evolutionary relationships among various species of fauna.
Microsatellites, also known as Simple Sequence Repeats (SSRs), are short, repetitive sequences of DNA (typically 1β6 base pairs) found throughout an organism's genome. They are highly polymorphic, meaning the number of repeats varies significantly between individuals and species. This high rate of mutation makes them excellent molecular markers for genetic studies.
In evolutionary biology, scientists use microsatellite DNA to:
- Trace lineage and kinship within and between populations.
- Measure genetic diversity and population structure.
- Reconstruct phylogenetic relationships to understand how different species of fauna evolved from common ancestors.
Options 2, 3, and 4 are incorrect because microsatellites are diagnostic tools for identification and mapping, not active agents for cellular transformation, plant propagation, or clinical drug metabolism testing.
PROVENANCE & STUDY PATTERN
Guest previewThis question bridges NCERT Class 12 Biology (Molecular Basis of Inheritance) and Environment Current Affairs (Tiger Census). While 'Microsatellite' sounds technical, it is the fundamental basis of 'DNA Fingerprinting' mentioned in every standard text. The strategy is to link the 'tool' (DNA profiling) with its 'component' (Microsatellites/STRs).
This question can be broken into the following sub-statements. Tap a statement sentence to jump into its detailed analysis.
- Statement 1: Is microsatellite DNA used to study evolutionary relationships among various species of fauna?
- Statement 2: Is microsatellite DNA used to stimulate stem cells to transform into diverse functional tissues?
- Statement 3: Is microsatellite DNA used to promote clonal propagation of horticultural plants?
- Statement 4: Is microsatellite DNA used to assess the efficacy of drugs when conducting drug trials in a population?
- Directly states microsatellite DNA analysis is useful to study genetic relationships between populations.
- Links microsatellite analysis to genetic/phylogenetic work in fish (fauna) contexts.
- Shows microsatellite DNA analyses being used to determine stock origin and population structure in Atlantic sturgeon (a faunal species).
- Compares microsatellite and mitochondrial DNA for estimating population structure and gene flow, indicating use in evolutionary/relationship studies.
- Describes molecular markers (a category that includes microsatellites) as tools for estimating population histories and genetic relationships within and between animal breeds.
- Links molecular markers to molecular characterization and determination of relationships in animals, supporting their use in evolutionary studies of fauna.
States that mitochondrial DNA studies provide information on prehistoric migrations and human dispersals, showing DNA markers are used to infer historical/evolutionary relationships.
A student can generalize that other genetic markers (e.g., microsatellites) may likewise be used to study evolutionary or population relationships among animals and check specialized literature on microsatellite applications.
Calls for DNA barcoding, a program to sequence standard gene regions and build reference libraries for species identification β an example of using DNA sequences to study species-level relationships.
A student could infer that different types of genetic markers (including short repetitive markers like microsatellites) are part of the toolbox for studying species relationships and population structure.
Describes DNA fingerprinting for individual identification of tigers from scats, demonstrating practical use of genetic markers in fauna studies and conservation.
From this, a student might extend that microsatellites β commonly used in DNA fingerprinting and population genetics β can be applied to study relationships and population structure in other animal species.
Explains DNA barcoding as sequencing standardized gene regions and comparing to a reference database to identify species β an explicit example of sequencing-based methods applied to biodiversity studies.
A student could reason that beyond barcoding loci, other genetic markers (such as microsatellites) serve complementary roles in resolving evolutionary and population-level questions.
Defines biodiversity to include genetic variation among organisms, highlighting that genetic data are central to understanding relationships among species and populations.
A student can use this principle to justify why molecular markers (including microsatellites) are relevant tools for studying evolutionary relationships among fauna.
Describes that stimulated cells can release chemical compounds which other cells detect and respond to β a general rule that chemical signals can change cell behaviour.
A student could extend this by noting microsatellite-derived factors would need to act like such signalling molecules or influence their production to steer stem cell differentiation.
Explains that DNA (genes) encodes proteins and that hormones (chemical signals) control traits like growth β linking DNA sequence to production of molecules that change cell fate.
One could infer that if microsatellite DNA affects gene expression, it might alter production of proteins/hormones that direct stem cells toward specific tissue types.
States multicellular organisms use specialised cell types and tissues β a pattern showing that distinct molecular programs yield specialised functions.
A student could reason that stimulating stem cells must change their molecular program to produce specialised tissues, so any candidate (e.g., microsatellite DNA) would need to influence those programs.
Notes existence of specialised cell lineages with different chromosome/DNA content for particular functions, illustrating that DNA configuration relates to specialized cell roles.
This supports extending the idea that alterations in DNA or its regulatory regions could be implicated in directing cells to particular fates, a prerequisite for claims about microsatellite involvement.
Points out DNA copying is imperfect and generates variations β a general example that DNA sequence differences can lead to different cellular outcomes.
A student might use this to reason that sequence repeats (microsatellites) could influence gene function/variation and thus be hypothesised to affect differentiation, warranting experimental testing.
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Explains vegetative propagation produces plants genetically similar to the parent, i.e., clonal propagation is used in horticulture (sugarcane, roses, grapes).
A student could infer that genetic markers (like microsatellites) might be useful to identify or verify these clonally propagated plants or to select parent material for propagation.
Describes tissue culture as a technique to grow many plants from one parent in disease-free conditions, a common method for producing multiple identical horticultural plants.
One could extend this to ask whether molecular markers (e.g., microsatellites) are used to monitor genetic fidelity or to screen for somaclonal variation among tissue-cultured clones.
Notes vegetative propagation is practised for growing some types of plants, highlighting the horticultural reliance on asexual/clonal methods.
This suggests testing whether molecular tools are applied in horticulture to confirm clonality or to select propagules, raising the possibility microsatellite markers could be used for that purpose.
Gives a concrete example (sisal) where propagation is done by bulbils and suckers because seed set is rare β an instance of routine clonal propagation in crops.
A student could reason that for crops propagated asexually, genetic assays (such as microsatellites) may be employed to track genetic identity or variability in propagules.
Mentions genetically modified crops as plants whose DNA has been modified using genetic engineering techniques, linking horticulture to DNA-level interventions.
From this one might extend to consider other DNA-based techniques (like molecular marker analysis) being applied in horticulture to support propagation or breeding decisions.
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Mentions "DNA fingerprinting" as a method to identify individuals from biological samples, showing that molecular genetic markers are used to track individuals/populations.
A student could infer that if genetic markers can identify individuals, similar markers (e.g., microsatellites) might be used to track genetic-related outcomes or stratify subjects in drug trials and then check specialized sources on pharmacogenetic markers.
Describes "genetic analysis to estimate tiger populations from faecal samples," indicating genetics is applied to monitor populations noninvasively.
A student could reason that genetic assays used to monitor populations could be adapted to monitor biological responses in human cohorts during trials and then look for literature on markers used in clinical studies.
Explains DNA barcoding and sequencing standardized gene regions for identification, demonstrating use of short DNA regions as reliable markers.
A student could extend this to ask whether short repetitive markers (like microsatellites) are similarly standardized for tracking genetic variation relevant to drug response and search pharmacogenetics resources.
Defines archaeogenetics as use of molecular genetics to study population history, showing that genetic marker analyses are standard tools in population-level studies.
A student could use this pattern to hypothesize that population-level genetics (including microsatellites) can be applied to study variability in drug response across populations and then verify in clinical genetics literature.
Discusses DNA copying errors as sources of variation in populations, highlighting that genetic variation exists and can be measured.
A student could combine this with knowledge that drug efficacy can vary with genetic differences and then investigate whether microsatellites are among markers used to correlate genotype with drug response.
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- [THE VERDICT]: Conceptual Sitter for Science students; Moderate for others. Source: NCERT Class 12 Biology, Chapter 6 (Human Genome Project & DNA Fingerprinting) + Tiger Census methodology.
- [THE CONCEPTUAL TRIGGER]: 'DNA Fingerprinting' and 'Genetic Markers' used in Wildlife Conservation (Project Tiger).
- [THE HORIZONTAL EXPANSION]: Memorize these Genetic Tools: 1) Microsatellites (STRs) = Paternity/Forensics/Population structure. 2) Minisatellites (VNTRs) = Older fingerprinting tech. 3) Mitochondrial DNA (mtDNA) = Maternal lineage/Evolutionary tracking. 4) SNPs (Single Nucleotide Polymorphisms) = Drug response/Precision medicine. 5) eDNA (Environmental DNA) = Detecting species from water/soil samples.
- [THE STRATEGIC METACOGNITION]: When you read 'Tiger Census uses DNA fingerprinting from scat,' ask 'What exactly in the DNA is being measured?' The answer is Microsatellites. UPSC tests the *mechanism* behind the *headline*.
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DNA barcoding is a standardized short-gene sequencing method used to identify species rapidly and accurately.
High-yield for questions on biodiversity inventory and conservation policy because it links molecular techniques to species-level identification and global initiatives like building reference libraries; connects to bioinformatics and national biodiversity programmes and enables questions on technological tools for conservation.
- Environment, Shankar IAS Acedemy .(ed 10th) > Chapter 16: Conservation Efforts > 16.17. DNA BARCODING > p. 248
- Environment, Shankar IAS Acedemy .(ed 10th) > Chapter 16: Conservation Efforts > Goals: > p. 249
Mitochondrial DNA is used to infer prehistoric migrations and maternal lineage relationships.
Important for topics on human migrations, evolutionary history and phylogeography; helps answer questions that connect genetics with historical demography and patterns of dispersal.
- History , class XI (Tamilnadu state board 2024 ed.) > Chapter 1: Early India: From the Beginnings to the Indus Civilisation > Sources > p. 1
DNA fingerprinting enables identification of individual animals from biological samples and aids population estimation.
Directly relevant to conservation practice and wildlife census methods (camera trapping vs genetic identification); useful for questions on methods to estimate and monitor endangered species and forensics in wildlife protection.
- Environment, Shankar IAS Acedemy .(ed 10th) > Chapter 16: Conservation Efforts > 16.r.3. Estimation of Tiger Populations > p. 229
- Environment and Ecology, Majid Hussain (Access publishing 3rd ed.) > Chapter 4: BIODIVERSITY > Ex-situ conservation > p. 30
Multicellular organisms form specialised cell types that are organised into tissues and organs, which is the outcome targeted when converting stem cells into functional tissues.
High-yield for questions on development, regeneration and reproductive biology; links cellular differentiation to physiology and organ-level function. Mastery helps answer questions on how cellular-level manipulations (like stem-cell work) must respect tissue organisation and specialised roles.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > How do Organisms Reproduce? CHAPTER7 > p. 116
- Science , class X (NCERT 2025 ed.) > Chapter 5: Life Processes > 5.1 WHAT ARE LIFE PROCESSES? > p. 80
Genes encode proteins that control cellular functions and hormones can trigger growth and physiological responses relevant to driving cell behaviour and differentiation.
Essential for genetics, endocrinology and developmental biology questions; explains mechanisms by which molecular signals can direct cell fate and tissue formation. Useful for evaluating interventions that aim to change cell behaviour by altering signalling or gene expression.
- Science , class X (NCERT 2025 ed.) > Chapter 8: Heredity > 8.2.3 How do these Traits get Expressed? > p. 131
- Science , class X (NCERT 2025 ed.) > Chapter 6: Control and Coordination > Activity 6.2 > p. 108
Accurate DNA copying, chromosome complement and the generation of variation underlie cellular identity and stability, which constrain how cells can be manipulated or reprogrammed.
Core for genetics and reproduction topics; helps reason about limits of genetic interventions, stability of engineered cells, and inheritance of cellular properties. Prepares candidates to connect molecular fidelity to larger biological outcomes.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.1 DO ORGANISMS CREATE EXACT COPIES OF THEMSEL THEMSELVES? > p. 114
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.3.1 Why the Sexual Mode of Reproduction? > p. 120
- Science , class X (NCERT 2025 ed.) > Chapter 8: Heredity > separate traits, shape and colour of seeds Figure 8.5 > p. 132
Vegetative propagation reproduces plants from roots, stems or leaves to produce genetically similar (clonal) plants.
High-yield topic for horticulture questions: explains how clonal uniformity, early flowering and propagation of seedless cultivars are achieved. Connects to crop-management, nursery practices and distinctions between sexual vs asexual reproductionβuseful for questions on propagation methods and advantages/disadvantages.
- Science , class X (NCERT 2025 ed.) > Chapter 7: How do Organisms Reproduce? > 7.2.5 Vegetative Propagation > p. 117
- Science ,Class VIII . NCERT(Revised ed 2025) > Chapter 13: Our Home: Earth, a Unique Life Sustaining Planet > 13.5.1 Asexual reproduction > p. 220
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Next Logical Question: 'Environmental DNA (eDNA)'. Since they asked about Microsatellites (tissue/scat analysis), the next step is eDNA (detecting species presence from water/soil without physical samples), which is currently revolutionizing biodiversity monitoring.
Etymology Hack: 'Satellite' in genetics refers to highly repetitive DNA. Repetitive things are unique to individuals/lineages (like fingerprints). Option B (Stem cells) and D (Drug trials) usually involve functional genes (coding regions), not repetitive 'junk' DNA. Option C is about growing plants. Option A (Evolutionary relationships) is the only one dealing with 'tracking lineage' or 'identity,' which is what fingerprints do.
Mains GS-3 (Science & Tech + Environment): Use this to argue for 'Non-invasive Conservation Technologies.' Genetic monitoring allows tracking of endangered species (like Cheetahs in Kuno) without the stress of physical capture.
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