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Consider the following statements about genetic engineering :
1. The technique of genetic engineering involves creation of recombinant DNA, use of gene cloning and gene transfer.
2. Restriction enzymes cut DNA at specific locations.
3. Restriction enzymes split the entire double-stranded DNA into two strands.
Which of the statements given above is/are correct?
Explanation
Statement 1 is correct: Genetic engineering (recombinant DNA technology) involves techniques like the creation of recombinant DNA, gene cloning, and gene transfer to overcome the limitations of traditional hybridization and introduce only desirable genes.
Statement 2 is correct: Restriction enzymes, known as "molecular scissors," are used to cut DNA at specific recognition sequences (palindromic sequences), which is a fundamental step in biotechnology.
Statement 3 is incorrect: Restriction enzymes do not split the double-stranded DNA into two separate single strands; that is the role of the enzyme DNA helicase during processes like replication. Instead, restriction enzymes cut the phosphodiester backbone of both strands of the double helix at specific points within their recognition sites. They may produce "sticky" or "blunt" ends, but the strands remain paired except at the point of the cut.