The correct answer is option 3. Statement 1 and 3 are correct.

Statement 1 says that our muscles ache after performing vigorous exercises because there is a relative deficit of ATP in muscle tissue. ATP is the energy currency of cells, including muscle cells. During vigorous exercise, our muscles require a lot of energy, and ATP is rapidly utilized. If ATP production cannot keep up with demand, there can be a relative deficit of ATP in the muscle tissue. This can result in muscle fatigue and ache.

Statement 2 is incorrect. It states that there is a total absence of ATP in muscle tissue, which is not true. While ATP levels may decrease during vigorous exercise, it is not completely absent.

Statement 3 is correct. It states that there is an excessive accumulation of lactic acid in the tissues. During intense exercise, the breakdown of glucose for energy can result in the production of lactic acid. This accumulation of lactic acid can contribute to muscle fatigue and ache.

Statement 4 is incorrect. It states that there is an ionic imbalance in the tissues. While there can be changes in ion concentrations during exercise, it is not the primary cause of muscle ache.

In summary, the correct statements are 1 and 3 – there is a relative deficit