The Fifty-second Constitutional Amendment Act focuses on provisions regarding disqualification of members of State Legislatures in the event of defection. It is important to understand the different provisions mentioned in the options:

Option 1: This provision states that if a member of the State Legislature gives up the membership of the party on whose ticket they were elected, they may face disqualification.

Option 2: The correct answer, this provision states that if a member is elected as a presiding officer of the House and gives up their party membership to become non-partisan, it is not considered a ground for disqualification.

Option 3: This provision states that if an independent member of the State Legislature joins any political party after the election, they may face disqualification.

Option 4: This provision states that if a member abstains from voting or votes contrary to any direction of the political party to which they belong, they may face disqualification.

In summary, the correct answer is option 2. It is important to note that the other options are all provisions mentioned in the Fifty-second Constitutional Amendment Act regarding disqualification of members of State Legislatures in the event of defection.