The Election Commission of India has various powers and functions related to conducting elections in the country. The options provided in the question are as follows:

Option 1: Superintendence, direction, and control of the preparation of electoral rolls.

This is one of the powers and functions of the Election Commission. It is responsible for overseeing the process of creating and updating electoral rolls.

Option 2: Conduct of elections to the Parliament and to the Legislature of each State.

This is another power and function of the Election Commission. It is responsible for conducting elections to the national Parliament as well as the state legislatures.

Option 3: Conduct of election to the office of the President and the Vice President.

This is also a power and function of the Election Commission. It conducts the election for the positions of the President and Vice President of India.

Option 4: Appointment of the Regional Commissioners to assist the Election Commission in the performance of the functions conferred on the Commission.

This option is not under the powers and functions of the Election Commission. The Election Commission does not have the authority to appoint Regional Commissioners to assist them in their work.

Therefore, option 4 is the correct answer as it is not a power or function of the Election Commission.