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The correct answer is option 3: "2, 3, and 4". Each of these factors contributes to the overall health and development of the savanna ecosystem.
2. Fire: Fires, both natural and man-made, regularly sweep through savannas, burning grasses and preventing the establishment and growth of trees.
3. Grazing Herbivores: Herbivores such as elephants, zebras, and gazelles, by eating the young shoots and leaves, prevent the establishment of a dense tree layer.
4. Seasonal Rainfall: Savannas have a wet-dry cycle. During the dry season, lack of water hampers tree growth, and trees that do grow cannot establish a large canopy.
Option 1 and 5 in option 4, i.e., "Burrowing animals and termites" and "Soil properties" respectively, are important ecosystem elements and contribute to overall structure and health, but they do not necessarily limit tree growth directly. Hence, only factors 2, 3 and 4 from option 3 have been chosen in the correct answer.