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The correct answer is option 4 - Ribosomes.
The rough endoplasmic reticulum (ER) gets its rough appearance because it has ribosomes present on its surface. Ribosomes are tiny structures that are responsible for protein synthesis. They are often found on the surface of the rough ER and are attached to it.
The endoplasmic reticulum is a complex network of interconnected tubes and sacs that are involved in various cellular processes. There are two types of endoplasmic reticulum - rough ER and smooth ER. The rough ER gets its name because it appears rough under a microscope due to the presence of ribosomes.
Ribosomes are responsible for translating the genetic information from the DNA into proteins. They read the messenger RNA (mRNA) and assemble the corresponding amino acids to form a polypeptide chain, which eventually folds into a functional protein.
Therefore, the rough endoplasmic reticulum is rough because of the presence of ribosomes, which are involved in protein synthesis.