The question asks which painter was not associated with Humayun. To better understand the answer, let`s look at each option.

1) Mir Sayyid Ali: Mir Sayyid Ali was a prominent painter during the Mughal period and he was indeed associated with Humayun. Therefore, option 1 is not the correct answer.

2) Maulana Dost Musawir: Maulana Dost Musawir was a renowned Persian artist of the 16th century. There is no mention of him being associated with Humayun, so it is possible that option 2 is the correct answer.

3) Maulana Yusuf: Similarly, there is no clear association mentioned between Maulana Yusuf and Humayun. Therefore, option 3 could be the correct answer.

4) Bihzad: Bihzad was a Persian miniature painter who lived during the Timurid period. He was known for his exceptional skills and was not associated with Humayun. Hence, option 4 is the correct answer.

Based on the information provided, option 4, Bihzad, is the painter who was not associated with Humayun.