The blotting technique used to identify the isolated protein is

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Q: 216 (SSC/NA)
The blotting technique used to identify the isolated protein is

question_subject: 

Science

question_exam: 

SSC

stats: 

0,5,26,8,5,8,10

keywords: 

{'isolated protein': [1, 0, 0, 0], 'northern blotting': [1, 0, 1, 1], 'southern blotting': [1, 0, 1, 0], 'blotting technique': [1, 0, 0, 0], 'western blotting': [1, 0, 0, 0], 'cloning': [0, 0, 0, 1]}

The correct answer is option 2: Western blotting. Western blotting is a technique used to detect and identify specific proteins in a sample. It involves separating the proteins based on their size using gel electrophoresis and then transferring them onto a membrane. The membrane is then incubated with antibodies that specifically bind to the target protein. The bound antibodies are then visualized using a colorimetric or fluorescent detection method. This technique is commonly used in research and diagnostics to determine the presence and quantity of a specific protein in a sample.

Option 1, Northern blotting, is used to detect specific RNA molecules.

Option 3, Southern blotting, is used to detect specific DNA molecules.

Option 4, Cloning, is a technique used to create genetically identical copies of a DNA sequence.

Therefore, the correct answer is option 2, Western blotting.

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